Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.

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Basrur V, Yang F, Kushimoto T, Higashimoto Y, Yasumoto K, Valencia J, Muller J, Vieira WD, Watabe H, Shabanowitz J, Hearing VJ, Hunt DF, Appella E

Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.

J Proteome Res. 2003 Jan-Feb;2(1):69-79.

PubMed ID
12643545 [ View in PubMed
]
Abstract

Melanin is a heterogeneous biopolymer produced only by specific cells termed melanocytes, which synthesize and deposit the pigment in specialized membrane-bound organelles known as melanosomes. Although melanosomes have been suspected of being closely related to lysosomes and platelets, the total number of melanosomal proteins is still unknown. Thus far, six melanosome-specific proteins have been identified, and the challenge is to characterize the complete proteome of the melanosome to further understand its mechanism of biogenesis. In this report, we used mass spectrometry and subcellular fractionation to identify protein components of early melanosomes. Using this approach, we have identified all 6 of the known melanosome-specific proteins, 56 proteins that are shared with other organelles, and confirmed the presence of 6 novel melanosomal proteins using western blotting and by immunohistochemistry.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
TyrosinaseP14679Details
Gamma-glutamyl hydrolaseQ92820Details
Cathepsin BP07858Details
Cathepsin DP07339Details
CalnexinP27824Details
78 kDa glucose-regulated proteinP11021Details
EndoplasminP14625Details
Protein disulfide-isomeraseP07237Details
Transmembrane glycoprotein NMBQ14956Details
V-type proton ATPase subunit B, brain isoformP21281Details
Ras-related protein Rab-7aP51149Details
Protein disulfide-isomerase A3P30101Details
V-type proton ATPase 116 kDa subunit a isoform 1Q93050Details
Melanocyte protein PMELP40967Details