Isolation of galactosyltransferase from human milk and the determination of its N-terminal amino acid sequence.
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Appert HE, Rutherford TJ, Tarr GE, Thomford NR, McCorquodale DJ
Isolation of galactosyltransferase from human milk and the determination of its N-terminal amino acid sequence.
Biochem Biophys Res Commun. 1986 Jul 16;138(1):224-9.
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- 3091013 [ View in PubMed]
- Abstract
Galactosyltransferase (EC 2.4.1.22), purified to homogeneity from human milk by affinity chromatography, had an apparent molecular weight of 53,000 as determined by denaturing polyacrylamide gel electrophoresis. Subtraction of the estimated contribution of the oligosaccharide portion of the molecule leaves a Mr of 47,000. An N-terminal amino acid sequence analysis of the isolated protein revealed a sequence similar to that found near the 5' end of a cDNA clone isolated by Shaper et al, which encodes a 35,000 molecular weight protein. Either the molecular weight of galactosyltransferase, has been overestimated, or a discrepancy exists between the actual molecular weight of galactosyltransferase and that predicted by the bovine cDNA clone isolated by Shaper et al.