Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor.
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Kobilka BK, Matsui H, Kobilka TS, Yang-Feng TL, Francke U, Caron MG, Lefkowitz RJ, Regan JW
Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor.
Science. 1987 Oct 30;238(4827):650-6.
- PubMed ID
- 2823383 [ View in PubMed]
- Abstract
The gene for the human platelet alpha 2-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the purified receptor. The identity of this gene has been confirmed by the binding of alpha 2-adrenergic ligands to the cloned receptor expressed in Xenopus laevis oocytes. The deduced amino acid sequence is most similar to the recently cloned human beta 2- and beta 1-adrenergic receptors; however, similarities to the muscarinic cholinergic receptors are also evident. Two related genes have been identified by low stringency Southern blot analysis. These genes may represent additional alpha 2-adrenergic receptor subtypes.