Purification of CpG islands using a methylated DNA binding column.
Article Details
- CitationCopy to clipboard
Cross SH, Charlton JA, Nan X, Bird AP
Purification of CpG islands using a methylated DNA binding column.
Nat Genet. 1994 Mar;6(3):236-44.
- PubMed ID
- 8012384 [ View in PubMed]
- Abstract
CpG islands are short stretches of DNA containing a high density of non-methylated CpG dinucleotides, predominantly associated with coding regions. We have constructed an affinity matrix that contains the methyl-CpG binding domain from the rat chromosomal protein MeCP2, attached to a solid support. A column containing the matrix fractionates DNA according to its degree of CpG methylation, strongly retaining those sequences that are highly methylated. Using this column, we have developed a procedure for bulk isolation of CpG islands from human genomic DNA. As CpG islands overlap with approximately 60% of human genes, the resulting CpG island library can be used to isolate full-length cDNAs and to place genes on genomic maps.