Molecular cloning and primary structure of human 15-lipoxygenase.
Article Details
- CitationCopy to clipboard
Sigal E, Craik CS, Highland E, Grunberger D, Costello LL, Dixon RA, Nadel JA
Molecular cloning and primary structure of human 15-lipoxygenase.
Biochem Biophys Res Commun. 1988 Dec 15;157(2):457-64.
- PubMed ID
- 3202857 [ View in PubMed]
- Abstract
A full-length cDNA encoding 15-lipoxygenase has been isolated from a human reticulocyte cDNA library. The predicted primary structure of the enzyme exhibits a sequence similarity of 61% and 45% with human 5-lipoxygenase and the soybean lipoxygenase isoenzyme I, respectively. When all three lipoxygenases are aligned, there are two distinct regions of significant sequence identity including a cluster of five histidine residues conserved in all three lipoxygenases. Because histidines can serve as ligands for the enzymatically active iron, this region may be critical to enzymatic function. These results provide a basis for exploring functional domains of lipoxygenases.