Isolation and characterization of a gene encoding alpha-tubulin from Candida albicans.
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Daly S, Yacoub A, Dundon W, Mastromei G, Islam K, Lorenzetti R
Isolation and characterization of a gene encoding alpha-tubulin from Candida albicans.
Gene. 1997 Mar 18;187(2):151-8.
- PubMed ID
- 9099874 [ View in PubMed]
- Abstract
A gene encoding the alpha-tubulin of Candida albicans has been cloned and characterized. Nucleotide sequence analysis reveals the presence of an intron within the structural gene and predicts the synthesis of a polypeptide of 448 amino acid residues. Comparison of nucleotide and amino acid sequences with the Saccharomyces cerevisiae alpha-tubulin encoding genes shows a 75% homology and about 92% similarity respectively. In contrast to S. cerevisiae, C. albicans appears to possess only one gene for alpha-tubulin which is able to functionally complement a S. cerevisiae cold-sensitive tub1 mutant.