Cloning and expression in Escherichia coli of the cDNA encoding human cardiac troponin I.
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Armour KL, Harris WJ, Tempest PR
Cloning and expression in Escherichia coli of the cDNA encoding human cardiac troponin I.
Gene. 1993 Sep 15;131(2):287-92.
- PubMed ID
- 8406024 [ View in PubMed]
- Abstract
We have cloned and sequenced the human cardiac troponin I (cTnI)-encoding cDNA with the aim of expressing the cDNA in Escherichia coli. The cDNA was successfully expressed as a fusion product with beta-galactosidase and as an unfused protein. Both polypeptides were recognised by an anti-human cTnI antibody.