Cloning and characterization of a glutamate transporter cDNA from human brain and pancreas.
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Manfras BJ, Rudert WA, Trucco M, Boehm BO
Cloning and characterization of a glutamate transporter cDNA from human brain and pancreas.
Biochim Biophys Acta. 1994 Oct 12;1195(1):185-8.
- PubMed ID
- 7522567 [ View in PubMed]
- Abstract
L-Glutamate is the major excitatory neurotransmitter in the brain. Sufficient removal from the synaptic cleft after neurotransmission by the L-glutamate transport system is essential to prevent excitotoxicity and neurotoxicity. We isolated mRNA from human brain and pancreatic islet cells and screened for sequences of high homology to a previously characterized rat brain glutamate transporter. An isolated sequence (GLTR) shows a 87.5% and a 92.5% sequence similarity at the nucleotide and amino acid level, respectively, with a rat brain specific L-glutamate transporter but only a 65% homology to the recently cloned human glutamate/aspartate transporter. The human mRNA is differentially expressed in brain and to a lesser degree in pancreas and in fetal liver. The gene encoding for the newly identified cDNA is located on chromosome 5.