On the relationship between human interferon alpha 1 and beta 1 genes.
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May LT, Sehgal PB
On the relationship between human interferon alpha 1 and beta 1 genes.
J Interferon Res. 1985 Summer;5(3):521-6.
- PubMed ID
- 2414376 [ View in PubMed]
- Abstract
The human interferon alpha 1 and beta 1 (IFN-alpha 1 and -beta 1) genes are approximately 56% identical in the nucleotide sequence across the regions coding for the mature proteins. An IFN-alpha 1 cDNA insert containing such sequences can be used to isolate 15 IFN-beta 1 cDNA colonies present in a 2600-strong library in Escherichia coli prepared from polyadenylated RNA extracted from human diploid fibroblasts (FS-4) induced with poly(I).poly(C). Conversely, a clone containing an IFN-alpha 1 cDNA exhibited detectable hybridization with an IFN-beta 1 probe. One of the IFN-beta 1 cDNA clones isolated in these experiments had lost the internal PstI and PvuII sites due to a change at nucleotide 277 from a C to an A. As a consequence, this allele codes for Met-47 instead of Leu-47. Suggestive evidence has been obtained for the presence of this allele in human genomic DNA (IFN-beta 1 Met-47).