Amino acid sequence of human liver cathepsin B.
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Ritonja A, Popovic T, Turk V, Wiedenmann K, Machleidt W
Amino acid sequence of human liver cathepsin B.
FEBS Lett. 1985 Feb 11;181(1):169-72.
- PubMed ID
- 3972105 [ View in PubMed]
- Abstract
The complete amino acid sequence of cathepsin B (EC 3.4.22.1) from human liver was determined. The 252-residue sequence was obtained by automated solid-phase Edman degradation of the light and heavy chain resulting from limited proteolysis of the single-chain enzyme and of fragments produced by cyanogen bromide and enzymatic cleavage of the heavy chain. Human liver cathepsin B has 83.7% identical residues with the corresponding enzyme from rat liver. Comparison of both mammalian cathepsin B sequences with the sequence of papain provides further evidence that lysosomal and plant cysteine proteinases have evolved from a common ancestor and share a similar catalytic mechanism.