Molecular analysis of thiopurine S-methyltransferase alleles in Taiwan aborigines and Taiwanese.
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Lu HF, Shih MC, Chang YS, Chang JY, Ko YC, Chang SJ, Chang JG
Molecular analysis of thiopurine S-methyltransferase alleles in Taiwan aborigines and Taiwanese.
J Clin Pharm Ther. 2006 Feb;31(1):93-8.
- PubMed ID
- 16476125 [ View in PubMed]
- Abstract
BACKGROUND: Thiopurine S-methyltransferase (TPMT) is a cytosolic enzyme involved in the metabolism of these thiopurine drugs. Methylation of thiopurine drugs by TPMT competes with the formation of their active 6-thioguanine nucleotide metabolite, thereby potentially modulating the therapeutic and toxic effects of these drugs. OBJECTIVE: To analyze the thiopurine S-methyltransferase allelic frequencies in Taiwan aborigines and Taiwanese. METHODS: We used polymerase chain reaction-restriction fragment length polymorphism method to determine the allelic frequencies of TPMT variants (TPMT*1-TPMT*8) in 409 Taiwan aborigines and 117 Taiwanese. RESULTS AND DISCUSSION: The results showed that the allelic frequencies of TPMT*1 were 99.88% and 98.72% for Taiwan aborigines and Taiwanese respectively. The allelic frequencies of TPMT*3C were 0.12% and 1.28% for Taiwan aborigines and Taiwanese respectively. No TPMT*2, 3A, 3B, 3D and 4-8 were found in these populations. CONCLUSION: Our results provide useful information for using thiopurine drugs in these populations.