Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli.
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Harris TJ, Patel T, Marston FA, Little S, Emtage JS, Opdenakker G, Volckaert G, Rombauts W, Billiau A, De Somer P
Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli.
Mol Biol Med. 1986 Jun;3(3):279-92.
- PubMed ID
- 3090401 [ View in PubMed]
- Abstract
cDNA clones of the mRNA coding for tissue-type plasminogen activator (t-PA) have been obtained and their nucleotide sequences compared to those reported previously. A gene coding for t-PA has been reconstructed and inserted into vectors for expression in prokaryotic cells. Relatively high levels of t-PA accumulated in inclusion bodies in Escherichia coli containing an optimized expression plasmid, but only a small proportion of the insoluble protein was recovered as active enzyme using a variety of solubilization procedures.