Three-dimensional atomic structure of a catalytic subunit mutant of human protein kinase CK2.
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Pechkova E, Zanotti G, Nicolini C
Three-dimensional atomic structure of a catalytic subunit mutant of human protein kinase CK2.
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2133-9. Epub 2003 Nov 27.
- PubMed ID
- 14646071 [ View in PubMed]
- Abstract
The three-dimensional crystal structure of the triple-point mutant of the catalytic subunit of human protein kinase CK2alpha has been determined at 2.4 A resolution. Microcrystals of mutant CK2 catalytic subunit were obtained by a protein-crystallization method based on thin-film nanotechnology. These microcrystals (of about 20 micro m in diameter) were used for diffraction data collection by means of the microfocus beamline at the ESRF synchrotron. A comparison between the human protein kinase CK2alpha and the corresponding enzyme from a lower organism (Zea mays) is made.