P-450 binding to substrates camphor and linalool versus pressure.
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Marden MC, Hoa GH
P-450 binding to substrates camphor and linalool versus pressure.
Arch Biochem Biophys. 1987 Feb 15;253(1):100-7.
- PubMed ID
- 3813557 [ View in PubMed]
- Abstract
The spin equilibrium of two bacterial cytochrome P-450 enzymes are compared by their visible spectra versus temperature and pressure. P-450 from Pseudomonas linalool shows a much weaker dependence on pressure than P-450 from P. putida which has camphor as substrate. The linalool system denatures at a higher pressure (3 kbar) than the camphor system (1 kbar) and shows a weaker dependence on external solvent conditions. The camphor system shows evidence of the binding of a second substrate molecule which reverses the effect of the first on the spin equilibrium. A model involving two substrate molecules is an alternative explanation of the apparent saturation with camphor of the spin equilibrium.