Crystallization and preliminary X-ray analysis of the periplasmic receptor (PotF) of the putrescine transport system in Escherichia coli.
Article Details
- CitationCopy to clipboard
Vassylyev DG, Kashiwagi T, Tomitori H, Kashiwagi K, Igarashi K, Morikawa K
Crystallization and preliminary X-ray analysis of the periplasmic receptor (PotF) of the putrescine transport system in Escherichia coli.
Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):132-4.
- PubMed ID
- 9761835 [ View in PubMed]
- Abstract
The primary receptor (PotF) of the putrescine transport system in E. coli has been crystallized by the hanging-drop vapor-diffusion technique. The crystals belong to the space group P21212 with unit-cell dimensions a = 269.4, b = 82.33 and c = 93.74 A. The crystals diffract beyond 2.2 A with a rotating-anode X-ray source. A complete data set from the native crystals has been collected and processed at 2.3 A resolution. Two heavy-atom derivatives have been prepared from the same Pt compound at 293 and 277 K. The difference Patterson maps revealed completely different major heavy-atom sites between these two derivatives.