Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp.
Article Details
- CitationCopy to clipboard
Ishizaki T, Hirayama N, Shinkawa H, Nimi O, Murooka Y
Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp.
J Bacteriol. 1989 Jan;171(1):596-601.
- PubMed ID
- 2914858 [ View in PubMed]
- Abstract
The nucleotide sequence of a 2.1-kilobase-pair fragment containing the Streptomyces choA gene, which codes a secreted cholesterol oxidase, was determined. A single open reading frame encodes a mature cholesterol oxidase of 504 amino acids, with a calculated Mr of 54,913. The leader peptides extend over 42 amino acids and have the characteristics of a signal sequence, including basic amino acids near the amino terminus and a hydrophobic core near the signal cleavage site. Analyses of the total amino acid composition and amino acid sequencing of the first 21 amino acids from the N terminus of the purified extracellular enzyme agree with the values deduced from nucleotide sequencing data.