Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum.
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Reddy SG, Scapin G, Blanchard JS
Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum.
Proteins. 1996 Aug;25(4):514-6.
- PubMed ID
- 8865347 [ View in PubMed]
- Abstract
The gene encoding the meso-diaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was over-expressed and purified to homogeneity. Crystals of the binary DAPDH-NADP+ complex were obtained from solutions of polyethylene glycol 8000, 100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc)2. The crystals diffract to 2.2 A, belong to the orthorhombic space group P2(1), and contain two molecules per asymmetric unit.