Characterization and crystallization of the helicase domain of bacteriophage T7 gene 4 protein.
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Bird LE, Hakansson K, Pan H, Wigley DB
Characterization and crystallization of the helicase domain of bacteriophage T7 gene 4 protein.
Nucleic Acids Res. 1997 Jul 1;25(13):2620-6.
- PubMed ID
- 9185573 [ View in PubMed]
- Abstract
Limited proteolysis of bacteriophage T7 primase/helicase with endoproteinase Glu-C produces several proteolytic fragments. One of these fragments, which is derived from the C-terminal region of the protein, was prepared and shown to retain helicase activity. This result supports a model in which the gene 4 proteins consist of functionally separable domains. Crystals of this C-terminal fragment of the protein have been obtained that are suitable for X-ray diffraction studies.