Characterization of the genes coding for the F1F0 subunits of the sodium dependent ATPase of Propionigenium modestum.
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Krumholz LR, Esser U, Simoni RD
Characterization of the genes coding for the F1F0 subunits of the sodium dependent ATPase of Propionigenium modestum.
FEMS Microbiol Lett. 1992 Feb 1;70(1):37-41.
- PubMed ID
- 1533602 [ View in PubMed]
- Abstract
The DNA coding for the eight structural genes and uncI of the sodium dependent ATPase of Propionigenium modestum has been cloned and sequenced. Based on sequence homology, the genes were determined to appear in the order uncBEFHAGDC as in several other bacterial species. Minicell experiments revealed that plasmids containing the P. modestum DNA expressed those ATPase polypeptides in Escherichia coli. These were very similar in molecular mass to those obtained from the purified ATPase of P. modestum. No membrane-bound ATPase activity was observed in E. coli unc deletion strains containing the P. modestum ATPase genes. Amino acid alignments which were done with the Fo subunits revealed only a few conservative changes in the highly conserved regions of the polypeptides.