Development of an ELISA for maduramicin and determination of the depletion kinetics of maduramicin residues in poultry.

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Kennedy DG, Blanchflower WJ, O'Dornan BC

Development of an ELISA for maduramicin and determination of the depletion kinetics of maduramicin residues in poultry.

Food Addit Contam. 1997 Jan;14(1):27-33.

PubMed ID
9059580 [ View in PubMed
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Abstract

Polyclonal antibodies against the coccidiostat maduramicin were produced by the immunization of rabbits with a conjugate between maduramicin and human serum albumin. The antibodies exhibited minimal cross-reactivity with structurally similar ionophoric coccidiostats (monensin, salinomycin, narasin and laslocid). An enzyme-linked immunosorbent assay (ELISA) was developed for maduramicin in poultry tissue using these antibodies. The ELISA had a limit of detection of approximately 0.02 ng/g in tissue (ng/ml in serum) and a limit of determination of 1.0 ng/g in tissue (ng/ml in serum). The half-life of maduramicin was measured in broiler chickens that had been treated with a therapeutic dose of maduramicin for 14 days. Following withdrawal of the medicated feed, the mean half-lives of maduramicin were 20, 39 and 13 h in liver, muscle and serum, respectively. At the recommended withdrawal period (5 days) the mean residual maduramicin concentration in liver was 1.6 ng/g, in muscle was 1.5 ng/g and in serum was 0.05 ng/ml.

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