The tyrosyl-tRNA synthetase from Escherichia coli. Complete nucleotide sequence of the structural gene.
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Barker DG, Bruton CJ, Winter G
The tyrosyl-tRNA synthetase from Escherichia coli. Complete nucleotide sequence of the structural gene.
FEBS Lett. 1982 Dec 27;150(2):419-23.
- PubMed ID
- 6761148 [ View in PubMed]
- Abstract
The structural component of the tyrS gene of Escherichia coli, comprising 1269 base pairs, has been fully sequenced by the combined M13/dideoxychain termination approach. The gene has a codon usage pattern which is typical of highly expressed proteins and similar to other Escherichia coli aminoacyl-tRNA synthetase genes. Peptide purification and sequencing has been used to locate the N-terminus and to provide confirmation of 95% of the translated protein sequence. This latter yields on Mr of 47,403 for the Escherichia coli tyrosyl-tRNA synthetase, and reveals considerable homology with the primary structure of the analogous enzyme isolated from Bacillus staerothermophilus.