Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli.
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Heller KB, Lin EC, Wilson TH
Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli.
J Bacteriol. 1980 Oct;144(1):274-8.
- PubMed ID
- 6998951 [ View in PubMed]
- Abstract
The specificity of the glycerol facilitator (glpF) of Escherichia coli was studied with an osmotic method. This transport system allowed the entry of polyols (glycerol and erythritol), pentitols, and hexitols. The analogous sugars were not transported. However, urea, glycine, and DL-glyceraldehyde could use this pathway to enter the cell. The glpF protein allowed the rapid efflux of preequilibrated xylitol. Glycerol surprisingly did not inhibit the uptake of xylitol, and xylitol only slightly reduced the uptake of glycerol. The observation and the insensitivity of the xylitol transport to low temperature suggest that the facilitator behaves as a membrane channel.