Thermonuclease
Details
- Name
- Thermonuclease
- Synonyms
- 3.1.31.1
- Micrococcal nuclease
- Staphylococcal nuclease
- TNase
- Gene Name
- nuc
- Organism
- Staphylococcus aureus
- Amino acid sequence
>lcl|BSEQ0003917|Thermonuclease MLVMTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSS ANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLL VDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKM VNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ
- Number of residues
- 231
- Molecular Weight
- 25471.16
- Theoretical pI
- 9.75
- GO Classification
- Functionsendonuclease activity / metal ion binding / nucleic acid bindingComponentsextracellular region / membrane
- General Function
- Nucleic acid binding
- Specific Function
- Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.
- Pfam Domain Function
- SNase (PF00565)
- Transmembrane Regions
- Not Available
- Cellular Location
- Secreted
- Gene sequence
>lcl|BSEQ0003916|696 bp GTGTTAGTTATGACAGAATACTTATTAAGTGCTGGCATATGTATGGCAATTGTTTCAATA TTACTTATAGGGATGGCTATCAGTAATGTTTCGAAAGGGCAATACGCAAAGAGGTTTTTC TTTTTCGCTACTAGTTGCTTAGTGTTAACTTTAGTTGTAGTTTCAAGTCTAAGTAGCTCA GCAAATGCATCACAAACAGATAACGGCGTAAATAGAAGTGGTTCTGAAGATCCAACAGTA TATAGTGCAACTTCAACTAAAAAATTACATAAAGAACCTGCGACTTTAATTAAAGCGATT GATGGTGATACGGTTAAATTAATGTACAAAGGTCAACCAATGACATTCAGACTATTATTG GTTGATACACCTGAAACAAAGCATCCTAAAAAAGGTGTAGAGAAATATGGTCCTGAAGCA AGTGCATTTACGAAAAAAATGGTAGAAAATGCAAAGAAAATTGAAGTCGAGTTTGACAAA GGTCAAAGAACTGATAAATATGGACGTGGCTTAGCGTATATTTATGCTGATGGAAAAATG GTAAACGAAGCTTTAGTTCGTCAAGGCTTGGCTAAAGTTGCTTATGTTTACAAACCTAAC AATACACATGAACAACATTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAAT ATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P00644 UniProtKB Entry Name NUC_STAAU GenBank Protein ID 673492 GenBank Gene ID V01281 - General References
- Shortle D: A genetic system for analysis of staphylococcal nuclease. Gene. 1983 May-Jun;22(2-3):181-9. [Article]
- Kovacevic S, Veal LE, Hsiung HM, Miller JR: Secretion of staphylococcal nuclease by Bacillus subtilis. J Bacteriol. 1985 May;162(2):521-8. [Article]
- Miller JR, Kovacevic S, Veal LE: Secretion and processing of staphylococcal nuclease by Bacillus subtilis. J Bacteriol. 1987 Aug;169(8):3508-14. [Article]
- Davis A, Moore IB, Parker DS, Taniuchi H: Nuclease B. A possible precursor of nuclease A, an extracellular nuclease of Staphylococcus aureus. J Biol Chem. 1977 Sep 25;252(18):6544-53. [Article]
- Cone JL, Cusumano CL, Taniuchi H, Anfinsen CB: Staphylococcal nuclease (Foggi strain). II. The amino acid sequence. J Biol Chem. 1971 May 25;246(10):3103-10. [Article]
- Bohnert JL, Taniuchi H: The examination of the presence of amide groups in glutamic acid and aspartic acid residues of staphylococcal nuclease (Foggi strain). J Biol Chem. 1972 Jul 25;247(14):4557-60. [Article]
- Cotton FA, Hazen EE Jr, Legg MJ: Staphylococcal nuclease: proposed mechanism of action based on structure of enzyme-thymidine 3',5'-bisphosphate-calcium ion complex at 1.5-A resolution. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2551-5. [Article]
- Hynes TR, Fox RO: The crystal structure of staphylococcal nuclease refined at 1.7 A resolution. Proteins. 1991;10(2):92-105. [Article]
- Wang JF, Mooberry ES, Walkenhorst WF, Markley JL: Solution studies of staphylococcal nuclease H124L. 1. Backbone 1H and 15N resonances and secondary structure of the unligated enzyme as identified by three-dimensional NMR spectroscopy. Biochemistry. 1992 Jan 28;31(3):911-20. [Article]
- Shortle D, Abeygunawardana C: NMR analysis of the residual structure in the denatured state of an unusual mutant of staphylococcal nuclease. Structure. 1993 Oct 15;1(2):121-34. [Article]