N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component
Details
- Name
- N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component
- Synonyms
- 2.7.1.69
- celA
- PTS system N,N'-diacetylchitobiose-specific EIIB component
- Gene Name
- chbB
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0016567|N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLAGEKGQNADVVLLGPQI AYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKKAAAN
- Number of residues
- 106
- Molecular Weight
- 11426.435
- Theoretical pI
- 8.45
- GO Classification
- Functionskinase activity / protein-N(PI)-phosphohistidine-sugar phosphotransferase activity / protein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activityProcessescarbohydrate transmembrane transport / N,N'-diacetylchitobiose import / phosphoenolpyruvate-dependent sugar phosphotransferase systemComponentscytosol
- General Function
- Protein-phosphocysteine-n,n'-diacetylchitobiose phosphotransferase system transporter activity
- Specific Function
- The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport.
- Pfam Domain Function
- PTS_IIB (PF02302)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0016568|N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component (chbB) ATGGAAAAGAAACACATTTATCTGTTTTGTTCTGCGGGCATGTCTACCTCTTTACTGGTA TCAAAAATGCGCGCACAGGCAGAAAAATATGAAGTTCCGGTCATTATTGAAGCATTTCCG GAAACACTGGCTGGTGAAAAAGGTCAGAATGCCGATGTCGTGTTATTAGGGCCGCAGATT GCTTATATGTTGCCCGAAATCCAGCGTTTGTTACCCAACAAACCGGTTGAAGTAATTGAC TCGCTGCTTTATGGCAAAGTCGATGGTTTAGGCGTGCTTAAGGCTGCGGTTGCAGCGATT AAAAAAGCCGCAGCAAATTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P69795 UniProtKB Entry Name PTQB_ECOLI GenBank Protein ID 41097 GenBank Gene ID X52890 - General References
- Parker LL, Hall BG: Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12. Genetics. 1990 Mar;124(3):455-71. [Article]
- Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.: A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):363-77. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Reizer J, Reizer A, Saier MH Jr: The cellobiose permease of Escherichia coli consists of three proteins and is homologous to the lactose permease of Staphylococcus aureus. Res Microbiol. 1990 Nov-Dec;141(9):1061-7. [Article]
- Keyhani NO, Roseman S: Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14367-71. [Article]
- Keyhani NO, Wang LX, Lee YC, Roseman S: The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system. J Biol Chem. 2000 Oct 20;275(42):33084-90. [Article]
- Keyhani NO, Bacia K, Roseman S: The transport/phosphorylation of N,N'-diacetylchitobiose in Escherichia coli. Characterization of phospho-IIB(Chb) and of a potential transition state analogue in the phosphotransfer reaction between the proteins IIA(Chb) AND IIB(Chb). J Biol Chem. 2000 Oct 20;275(42):33102-9. [Article]
- Keyhani N, Rodgers ME, Demeler B, Hansen JC, Roseman S: Analytical sedimentation of the IIAChb and IIBChb proteins of the Escherichia coli N,N'-diacetylchitobiose phosphotransferase system. Demonstration of a model phosphotransfer transition state complex. J Biol Chem. 2000 Oct 20;275(42):33110-5. [Article]
- Ab E, Schuurman-Wolters GK, Saier MH, Reizer J, Jacuinod M, Roepstorff P, Dijkstra K, Scheek RM, Robillard GT: Enzyme IIBcellobiose of the phosphoenol-pyruvate-dependent phosphotransferase system of Escherichia coli: backbone assignment and secondary structure determined by three-dimensional NMR spectroscopy. Protein Sci. 1994 Feb;3(2):282-90. [Article]
- Ab E, Schuurman-Wolters G, Reizer J, Saier MH, Dijkstra K, Scheek RM, Robillard GT: The NMR side-chain assignments and solution structure of enzyme IIBcellobiose of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli. Protein Sci. 1997 Feb;6(2):304-14. [Article]
- van Montfort RL, Pijning T, Kalk KH, Reizer J, Saier MH Jr, Thunnissen MM, Robillard GT, Dijkstra BW: The structure of an energy-coupling protein from bacteria, IIBcellobiose, reveals similarity to eukaryotic protein tyrosine phosphatases. Structure. 1997 Feb 15;5(2):217-25. [Article]