beta-Branched acyclic nucleoside analogues as inhibitors of Plasmodium falciparum dUTPase.
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Baragana B, McCarthy O, Sanchez P, Bosch-Navarrete C, Kaiser M, Brun R, Whittingham JL, Roberts SM, Zhou XX, Wilson KS, Johansson NG, Gonzalez-Pacanowska D, Gilbert IH
beta-Branched acyclic nucleoside analogues as inhibitors of Plasmodium falciparum dUTPase.
Bioorg Med Chem. 2011 Apr 1;19(7):2378-91. doi: 10.1016/j.bmc.2011.02.012. Epub 2011 Feb 17.
- PubMed ID
- 21411327 [ View in PubMed]
- Abstract
We report a series of beta-branched acyclic tritylated deoxyuridine analogues as inhibitors of Plasmodium falciparum deoxyuridine-5'-triphosphate nucleotidohydrolase (PfdUTPase), an enzyme involved in nucleotide metabolism that acts as first line of defence against uracil incorporation into DNA. Compounds were assayed against both PfdUTPase and intact parasites showing a correlation between enzyme inhibition and cellular assays. beta-Branched acyclic uridine analogues described here showed equal or slightly better potency and selectivity compared with previously reported analogues. The best inhibitor gave a K(i) of 0.5 muM against PfdUTPase with selectivity greater than 200-fold compared to the corresponding human enzyme and sub-micromolar growth inhibition of P. falciparum (EC(50) 0.6 muM). A crystal structure of the complex of PfdUTPase with one of the inhibitors shows that this acyclic derivative binds to the active site in a similar manner to that previously reported for a tritylated cyclic deoxyuridine derivative.