On the specificity of carboxypeptidase N, a comparative study.

Article Details

Citation

Hendriks D, Vingron M, Vriend G, Wang W, Nalis D, Scharpe S

On the specificity of carboxypeptidase N, a comparative study.

Biol Chem Hoppe Seyler. 1993 Sep;374(9):843-9.

PubMed ID
8267877 [ View in PubMed
]
Abstract

The structure of the enzymatically active subunit of human plasma carboxypeptidase N was modeled based on the homology with bovine carboxypeptidase A. The active site of carboxypeptidase N is well conserved in comparison with carboxypeptidase A. From a comparison of energetically favorable binding sites for different atomic probe groups a hypothesis for the differences in substrate specificity between carboxypeptidases A and N was derived. Small synthetic peptide substrates were synthesized to confirm this hypothesis. This study shows that even with very low homology model building by homology can be employed to build models of sufficient quality to aid in drug design.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Carboxypeptidase N catalytic chainP15169Details