Structure of the S15,S6,S18-rRNA complex: assembly of the 30S ribosome central domain.
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Agalarov SC, Sridhar Prasad G, Funke PM, Stout CD, Williamson JR
Structure of the S15,S6,S18-rRNA complex: assembly of the 30S ribosome central domain.
Science. 2000 Apr 7;288(5463):107-13.
- PubMed ID
- 10753109 [ View in PubMed]
- Abstract
The crystal structure of a 70-kilodalton ribonucleoprotein complex from the central domain of the Thermus thermophilus 30S ribosomal subunit was solved at 2.6 angstrom resolution. The complex consists of a 104-nucleotide RNA fragment composed of two three-helix junctions that lie at the end of a central helix, and the ribosomal proteins S15, S6, and S18. S15 binds the ribosomal RNA early in the assembly of the 30S ribosomal subunit, stabilizing a conformational reorganization of the two three-helix junctions that creates the RNA fold necessary for subsequent binding of S6 and S18. The structure of the complex demonstrates the central role of S15-induced reorganization of central domain RNA for the subsequent steps of ribosome assembly.