N-linked oligosaccharide structures in the diamine oxidase from porcine kidney.
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Huang Y, Mechref Y, Novotny MV
N-linked oligosaccharide structures in the diamine oxidase from porcine kidney.
Carbohydr Res. 2000 Jan 12;323(1-4):111-25.
- PubMed ID
- 10782293 [ View in PubMed]
- Abstract
Structures of the N-linked glycans released from porcine kidney diamine oxidase (DAO) were characterized utilizing various analytical techniques, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS), high-performance capillary electrophoresis (HPCE), and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The oligosaccharide sequences present in DAO were conclusively determined using specific exoglycosidases in conjunction with MALDI/TOF-MS. The structures found in the glycoprotein are primarily linear, di-, or tribranched fucosylated complex type. MS analysis of the esterified N-glycan pool derived from DAO indicated the presence of several di- and trisialylated structures.