Primary structure of bovine adrenal phenylethanolamine N-methyltransferase.
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Wong DL, Yoo YS, Lau K, Schilling JW
Primary structure of bovine adrenal phenylethanolamine N-methyltransferase.
Neuropsychopharmacology. 1990 Jun;3(3):175-80.
- PubMed ID
- 2363805 [ View in PubMed]
- Abstract
Bovine adrenal medullary phenylethanolamine N-methyltransferase (E.C. 2.1.1.28) was sequenced to determine if primary structure or post-translational processing accounts for the charged isozymes. A blocked NH2-terminus precluded amino terminal sequencing. Therefore, cyanogen bromide and tryptic peptide fragments were isolated and subjected to gas phase/gas-liquid phase sequencing. Primary structure was identified for 45% of the protein. At least two polypeptide chains exist with alternative amino acids representing conservative changes or single-base changes in amino acid codons by comparison to the cDNA sequence for the enzyme.