DBC1 (Deleted in Breast Cancer 1) modulates the stability and function of the nuclear receptor Rev-erbalpha.
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Chini CC, Escande C, Nin V, Chini EN
DBC1 (Deleted in Breast Cancer 1) modulates the stability and function of the nuclear receptor Rev-erbalpha.
Biochem J. 2013 May 1;451(3):453-61. doi: 10.1042/BJ20121085.
- PubMed ID
- 23398316 [ View in PubMed]
- Abstract
The nuclear receptor Rev-erbalpha has been implicated as a major regulator of the circadian clock and integrates circadian rhythm and metabolism. Rev-erbalpha controls circadian oscillations of several clock genes and Rev-erbalpha protein degradation is important for maintenance of the circadian oscillations and also for adipocyte differentiation. Elucidating the mechanisms that regulate Rev-erbalpha stability is essential for our understanding of these processes. In the present paper, we report that the protein DBC1 (Deleted in Breast Cancer 1) is a novel regulator of Rev-erbalpha. Rev-erbalpha and DBC1 interact in cells and in vivo, and DBC1 modulates the Rev-erbalpha repressor function. Depletion of DBC1 by siRNA (small interfering RNA) in cells or in DBC1-KO (knockout) mice produced a marked decrease in Rev-erbalpha protein levels, but not in mRNA levels. In contrast, DBC1 overexpression significantly enhanced Rev-erbalpha protein stability by preventing its ubiquitination and degradation. The regulation of Rev-erbalpha protein levels and function by DBC1 depends on both the N-terminal and C-terminal domains of DBC1. More importantly, in cells depleted of DBC1, there was a dramatic decrease in circadian oscillations of both Rev-erbalpha and BMAL1. In summary, our data identify DBC1 as an important regulator of the circadian receptor Rev-erbalpha and proposes that Rev-erbalpha could be involved in mediating some of the physiological effects of DBC1.