The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis.
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Wagner J, Gruz P, Kim SR, Yamada M, Matsui K, Fuchs RP, Nohmi T
The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis.
Mol Cell. 1999 Aug;4(2):281-6. doi: 10.1016/s1097-2765(00)80376-7.
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- 10488344 [ View in PubMed]
- Abstract
In Escherichia coli, the dinB gene is required for the SOS-induced lambda untargeted mutagenesis pathway and confers a mutator phenotype to the cell when the gene product is overexpressed. Here, we report that the purified DinB protein is a DNA polymerase. This novel E. coli DNA polymerase (pol IV) is shown to be strictly distributive, devoid of proofreading activity, and prone to elongate bulged (misaligned) primer/template structures. Site-directed mutagenesis experiments of dinB also demonstrate that the polymerase activity of DinB is required for its in vivo mutagenicity. Along with the sequence homologies previously found within the UmuC-like protein family, these results indicate that the uncovered DNA polymerase activity may be a common feature of all these homologous proteins.