DNA polymerase IV

Details

Name
DNA polymerase IV
Synonyms
  • 2.7.7.7
  • dinP
  • Pol IV
  • Translesion synthesis polymerase IV
  • TSL polymerase IV
Gene Name
dinB
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0052707|DNA polymerase IV
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTG
MALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSAT
LIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQFVITPAEVPAFLQTLPLAK
IPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQ
EHVWPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLLDPQMERQLVLGL
Number of residues
351
Molecular Weight
39515.52
Theoretical pI
Not Available
GO Classification
Functions
damaged DNA binding / DNA-directed DNA polymerase activity / magnesium ion binding
Processes
cellular response to DNA damage stimulus / DNA synthesis involved in DNA repair / DNA-dependent DNA replication / error-free translesion synthesis / error-prone translesion synthesis / SOS response
Components
cytoplasm
General Function
Poorly processive, error-prone DNA polymerase involved in translesion repair and untargeted mutagenesis (PubMed:10488344, PubMed:10801133). Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by Pol IV. Exhibits no 3'-5' exonuclease (proofreading) activity (PubMed:10488344). Overexpression of Pol IV results in increased frameshift mutagenesis. It is required for stationary-phase adaptive mutation, which provides the bacterium with flexibility in dealing with environmental stress, enhancing long-term survival and evolutionary fitness. Not seen to be involved in translesion snythesis even when stimulated by the beta slding-clamp and clamp-loading complex, which do however increase non-targeted DNA polymerase efficiency 3,000-fold, may be due to targeting to stalled replication forks on nondamaged DNA (PubMed:10801133, PubMed:16168375). Involved in translesional synthesis, in conjunction with the beta clamp from PolIII (PubMed:14592985, PubMed:14729336).
Specific Function
Damaged dna binding
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0052708|DNA polymerase IV (dinB)
ATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGCCGCAGTGGAGATGCGCGAC
AATCCCGCCCTGCGCGATATCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTG
ATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGG
ATGGCGCTCAAATTATGCCCACATCTCACCTTGCTTCCGGGGCGCTTTGACGCCTACAAA
GAAGCCTCAAATCATATCCGTGAAATCTTCTCGCGCTACACCTCGCGCATTGAACCGTTG
TCACTGGATGAGGCTTATCTCGATGTCACCGATAGCGTCCATTGCCACGGTTCTGCGACC
CTCATCGCCCAGGAAATCCGCCAGACAATCTTCAACGAGCTGCAACTGACGGCGTCTGCG
GGCGTGGCACCAGTAAAGTTTCTCGCCAAAATCGCCTCCGACATGAATAAACCCAACGGC
CAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTTTACAAACCTTACCGCTGGCAAAA
ATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGC
GGTGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTGGCAAATTTGGCCGC
ATTTTGTGGGAGCGTAGTCAGGGGATTGACGAACGCGATGTTAACAGCGAACGGTTGCGA
AAATCCGTCGGCGTGGAACGCACGATGGCGGAAGATATTCATCACTGGTCTGAATGTGAA
GCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGAT
TTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAG
GAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGG
GATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGAC
CCGCAAATGGAAAGACAACTGGTGCTGGGATTATGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDQ47155
UniProtKB Entry NameDPO4_ECOLI
General References
  1. Ohmori H, Hatada E, Qiao Y, Tsuji M, Fukuda R: dinP, a new gene in Escherichia coli, whose product shows similarities to UmuC and its homologues. Mutat Res. 1995 Jun;347(1):1-7. doi: 10.1016/0165-7992(95)90024-1. [Article]
  2. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  3. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  4. Kenyon CJ, Walker GC: DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli. Proc Natl Acad Sci U S A. 1980 May;77(5):2819-23. doi: 10.1073/pnas.77.5.2819. [Article]
  5. Kim SR, Maenhaut-Michel G, Yamada M, Yamamoto Y, Matsui K, Sofuni T, Nohmi T, Ohmori H: Multiple pathways for SOS-induced mutagenesis in Escherichia coli: an overexpression of dinB/dinP results in strongly enhancing mutagenesis in the absence of any exogenous treatment to damage DNA. Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13792-7. doi: 10.1073/pnas.94.25.13792. [Article]
  6. Wagner J, Gruz P, Kim SR, Yamada M, Matsui K, Fuchs RP, Nohmi T: The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis. Mol Cell. 1999 Aug;4(2):281-6. doi: 10.1016/s1097-2765(00)80376-7. [Article]
  7. Napolitano R, Janel-Bintz R, Wagner J, Fuchs RP: All three SOS-inducible DNA polymerases (Pol II, Pol IV and Pol V) are involved in induced mutagenesis. EMBO J. 2000 Nov 15;19(22):6259-65. doi: 10.1093/emboj/19.22.6259. [Article]
  8. Tang M, Pham P, Shen X, Taylor JS, O'Donnell M, Woodgate R, Goodman MF: Roles of E. coli DNA polymerases IV and V in lesion-targeted and untargeted SOS mutagenesis. Nature. 2000 Apr 27;404(6781):1014-8. doi: 10.1038/35010020. [Article]
  9. McKenzie GJ, Lee PL, Lombardo MJ, Hastings PJ, Rosenberg SM: SOS mutator DNA polymerase IV functions in adaptive mutation and not adaptive amplification. Mol Cell. 2001 Mar;7(3):571-9. doi: 10.1016/s1097-2765(01)00204-0. [Article]
  10. Lenne-Samuel N, Wagner J, Etienne H, Fuchs RP: The processivity factor beta controls DNA polymerase IV traffic during spontaneous mutagenesis and translesion synthesis in vivo. EMBO Rep. 2002 Jan;3(1):45-9. doi: 10.1093/embo-reports/kvf007. Epub 2001 Dec 19. [Article]
  11. Yeiser B, Pepper ED, Goodman MF, Finkel SE: SOS-induced DNA polymerases enhance long-term survival and evolutionary fitness. Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8737-41. doi: 10.1073/pnas.092269199. Epub 2002 Jun 11. [Article]
  12. McKenzie GJ, Magner DB, Lee PL, Rosenberg SM: The dinB operon and spontaneous mutation in Escherichia coli. J Bacteriol. 2003 Jul;185(13):3972-7. doi: 10.1128/JB.185.13.3972-3977.2003. [Article]
  13. Indiani C, McInerney P, Georgescu R, Goodman MF, O'Donnell M: A sliding-clamp toolbelt binds high- and low-fidelity DNA polymerases simultaneously. Mol Cell. 2005 Sep 16;19(6):805-15. doi: 10.1016/j.molcel.2005.08.011. [Article]
  14. Friedberg EC, Fischhaber PL, Kisker C: Error-prone DNA polymerases: novel structures and the benefits of infidelity. Cell. 2001 Oct 5;107(1):9-12. doi: 10.1016/s0092-8674(01)00509-8. [Article]
  15. McKenzie GJ, Rosenberg SM: Adaptive mutations, mutator DNA polymerases and genetic change strategies of pathogens. Curr Opin Microbiol. 2001 Oct;4(5):586-94. doi: 10.1016/s1369-5274(00)00255-1. [Article]
  16. Davies BW, Kohanski MA, Simmons LA, Winkler JA, Collins JJ, Walker GC: Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia coli. Mol Cell. 2009 Dec 11;36(5):845-60. doi: 10.1016/j.molcel.2009.11.024. [Article]
  17. Goodman MF: Error-prone repair DNA polymerases in prokaryotes and eukaryotes. Annu Rev Biochem. 2002;71:17-50. doi: 10.1146/annurev.biochem.71.083101.124707. Epub 2001 Nov 9. [Article]
  18. Bunting KA, Roe SM, Pearl LH: Structural basis for recruitment of translesion DNA polymerase Pol IV/DinB to the beta-clamp. EMBO J. 2003 Nov 3;22(21):5883-92. [Article]
  19. Burnouf DY, Olieric V, Wagner J, Fujii S, Reinbolt J, Fuchs RP, Dumas P: Structural and biochemical analysis of sliding clamp/ligand interactions suggest a competition between replicative and translesion DNA polymerases. J Mol Biol. 2004 Jan 30;335(5):1187-97. doi: 10.1016/j.jmb.2003.11.049. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails