Effect of topical microbicides on infectious human immunodeficiency virus type 1 binding to epithelial cells.

Article Details

Citation
Copy to clipboard

Roth S, Monsour M, Dowland A, Guenthner PC, Hancock K, Ou CY, Dezzutti CS

Effect of topical microbicides on infectious human immunodeficiency virus type 1 binding to epithelial cells.

Antimicrob Agents Chemother. 2007 Jun;51(6):1972-8. Epub 2007 Apr 2.

PubMed ID
17404008 [ View in PubMed
]
Abstract

Topical microbicides (cellulose acetate 1,2 benzene dicarboxylate [CAP], PRO 2000, SPL7013, and UC781) are being investigated to reduce the sexual transmission of human immunodeficiency virus type 1 (HIV-1). These products were shown to prevent the transfer of infectious HIV-1 from urogenital and colorectal epithelial cell lines to peripheral blood mononuclear cells. However, it was unclear if the topical microbicides rendered the virus noninfectious and/or reduced the binding to the epithelial cells. To test this, epithelial cells were cultured with HIV-1 in the presence or absence of topical microbicides or their placebos. The cells were washed, RNA lysates were made, and real-time PCR was performed for HIV-1. PRO 2000 and SPL7013 significantly (P < 0.0001) reduced the amount of bound HIV-1 to the colorectal epithelial cell line across clades A, B, C, and CRF01-AE. While none of the products reduced the binding of HIV-1 clades A and C to the urogenital cell line, CAP, PRO 2000, and SPL7013 significantly (P

DrugBank Data that Cites this Article

Drugs