Rab26 modulates the cell surface transport of alpha2-adrenergic receptors from the Golgi.
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Li C, Fan Y, Lan TH, Lambert NA, Wu G
Rab26 modulates the cell surface transport of alpha2-adrenergic receptors from the Golgi.
J Biol Chem. 2012 Dec 14;287(51):42784-94. doi: 10.1074/jbc.M112.410936. Epub 2012 Oct 26.
- PubMed ID
- 23105096 [ View in PubMed]
- Abstract
The molecular mechanisms underlying the transport from the Golgi to the cell surface of G protein-coupled receptors remain poorly elucidated. Here we determined the role of Rab26, a Ras-like small GTPase involved in vesicle-mediated secretion, in the cell surface export of alpha(2)-adrenergic receptors. We found that transient expression of Rab26 mutants and siRNA-mediated depletion of Rab26 significantly attenuated the cell surface numbers of alpha(2A)-AR and alpha(2B)-AR, as well as ERK1/2 activation by alpha(2B)-AR. Furthermore, the receptors were extensively arrested in the Golgi by Rab26 mutants and siRNA. Moreover, Rab26 directly and activation-dependently interacted with alpha(2B)-AR, specifically the third intracellular loop. These data demonstrate that the small GTPase Rab26 regulates the Golgi to cell surface traffic of alpha(2)-adrenergic receptors, likely through a physical interaction. These data also provide the first evidence implicating an important function of Rab26 in coordinating plasma membrane protein transport.