Nucleotide sequence of a functional cDNA for human thymidylate synthase.
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Takeishi K, Kaneda S, Ayusawa D, Shimizu K, Gotoh O, Seno T
Nucleotide sequence of a functional cDNA for human thymidylate synthase.
Nucleic Acids Res. 1985 Mar 25;13(6):2035-43.
- PubMed ID
- 2987839 [ View in PubMed]
- Abstract
We have determined the nucleotide sequence of a cDNA clone, pcHTS-1, encoding human thymidylate synthase (5,10-methylenetetrahydrofolate: dUMP C-methyltransferase, EC 2.1.1.45) which was previously isolated from a human fibroblast expressible cDNA library and functional in mouse cells. The 1.6 kilobase cDNA insert of pcHTS-1 encodes a subunit protein of 313 amino acid (Mr = 35,706) and its predicted amino acid sequence is highly conserved in many regions including folylpolyglutamate and 5-fluoro-2'-deoxyuridylate binding sites, when compared with those of Lactobacillus casei, Escherichia coli, and bacteriophage T4. The cDNA contains in its 5'-untranslated region a triple tandemly repeated sequence consisting of 90 nucleotides, which starts immediately upstream of the ATG initiator codon, is very high in G+C content (80%), and can form three possible interconvertible stem-loop structures.