Cloning and expression of the human 5-HT1B-type receptor gene.
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Mochizuki D, Yuyama Y, Tsujita R, Komaki H, Sagai H
Cloning and expression of the human 5-HT1B-type receptor gene.
Biochem Biophys Res Commun. 1992 Jun 15;185(2):517-23.
- PubMed ID
- 1610347 [ View in PubMed]
- Abstract
We report the cloning and expression of a novel 5-HT receptor gene from human genomic DNA. This clone, HGCR1, contains an apparently intronless open reading frame of 390 amino acids with the seven hydrophobic regions, typical of G-protein coupled receptors. The deduced amino acid sequence of HGCR1 is 39%, 55% and 87% identical to that for the human 5-HT1A, the human 5-HT1D and the rat 5-HT1B receptor, respectively. [3H]5-HT binding to transfected COS-7 cell membranes yields a pharmacological profile similar to that of 5-HT1B receptor. Thus these findings indicate the presence of 5-HT1B-type receptor in the human.