DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.

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Burland V, Plunkett G 3rd, Daniels DL, Blattner FR

DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.

Genomics. 1993 Jun;16(3):551-61.

PubMed ID
7686882 [ View in PubMed
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Abstract

The DNA sequence of a 136-kb segment (81.5 to 84.5 min) from the Escherichia coli origin of replication region has been determined and analyzed. Of the 122 protein coding regions that were found, we could assign no gene name or function to half of them, even in this well-studied part of the genome. The newly sequenced region also includes five RNA genes. The arrangement of open reading frames and potential promoters suggests 63 transcription units. The sequence was also analyzed for bend sites and two types of repeated sequence elements. Together with our sequence of the 84.5 to 86.5 min region, this new determination forms a 227-kb contiguous region centered on oriC. A global analysis of this region reveals a remarkable symmetry: most genes are transcribed divergently from the replication origin, and Chi octanucleotide (5'GCTGGTGG3') recombinational hot spots are also strikingly oriented with respect to the directions of replication and translation.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
DNA gyrase subunit BP0AES6Details
Deoxyuridine 5'-triphosphate nucleotidohydrolaseP06968Details
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]P17169Details
Alpha-xylosidaseP31434Details
Ribose import binding protein RbsBP02925Details
RibokinaseP0A9J6Details
Coenzyme A biosynthesis bifunctional protein CoaBCP0ABQ0Details
Phosphate-binding protein PstSP0AG82Details
Bifunctional protein GlmUP0ACC7Details
ATP synthase subunit bP0ABA0Details
DNA polymerase III subunit betaP0A988Details