Human phosphatidylcholine transfer protein: purification, crystallization and preliminary X-ray diffraction data.
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Chan WW, Roderick SL, Cohen DE
Human phosphatidylcholine transfer protein: purification, crystallization and preliminary X-ray diffraction data.
Biochim Biophys Acta. 2002 Apr 1;1596(1):1-5.
- PubMed ID
- 11983415 [ View in PubMed]
- Abstract
We have expressed, purified and crystallized recombinant human phosphatidylcholine transfer protein (PC-TP) and selenomethionyl PC-TP bound to dilinoleoyl phosphatidylcholine. The biochemical properties of native and selenomethionyl PC-TP were indistinguishable, and the two proteins crystallized under similar conditions. Both native and selenomethionyl PC-TP crystallized in two distinct space groups and diffracted X-rays to 2.4 A resolution.