DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae.

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Heidelberg JF, Eisen JA, Nelson WC, Clayton RA, Gwinn ML, Dodson RJ, Haft DH, Hickey EK, Peterson JD, Umayam L, Gill SR, Nelson KE, Read TD, Tettelin H, Richardson D, Ermolaeva MD, Vamathevan J, Bass S, Qin H, Dragoi I, Sellers P, McDonald L, Utterback T, Fleishmann RD, Nierman WC, White O, Salzberg SL, Smith HO, Colwell RR, Mekalanos JJ, Venter JC, Fraser CM

DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae.

Nature. 2000 Aug 3;406(6795):477-83.

PubMed ID
10952301 [ View in PubMed
]
Abstract

Here we determine the complete genomic sequence of the gram negative, gamma-Proteobacterium Vibrio cholerae El Tor N16961 to be 4,033,460 base pairs (bp). The genome consists of two circular chromosomes of 2,961,146 bp and 1,072,314 bp that together encode 3,885 open reading frames. The vast majority of recognizable genes for essential cell functions (such as DNA replication, transcription, translation and cell-wall biosynthesis) and pathogenicity (for example, toxins, surface antigens and adhesins) are located on the large chromosome. In contrast, the small chromosome contains a larger fraction (59%) of hypothetical genes compared with the large chromosome (42%), and also contains many more genes that appear to have origins other than the gamma-Proteobacteria. The small chromosome also carries a gene capture system (the integron island) and host 'addiction' genes that are typically found on plasmids; thus, the small chromosome may have originally been a megaplasmid that was captured by an ancestral Vibrio species. The V. cholerae genomic sequence provides a starting point for understanding how a free-living, environmental organism emerged to become a significant human bacterial pathogen.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Cholera enterotoxin subunit BP01556Details
Uncharacterized proteinQ9KVB4Details
DevB proteinQ9KL51Details
Type II secretion system protein EP37093Details
Beta-hexosaminidaseQ9KU37Details
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidaseQ9KPI8Details
CAI-1 autoinducer synthaseQ9KM65Details