Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase.
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Anton IA, Coggins JR
Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase.
Biochem J. 1988 Jan 15;249(2):319-26.
- PubMed ID
- 3277621 [ View in PubMed]
- Abstract
The Escherichia coli aroE gene encoding shikimate dehydrogenase was sequenced. The deduced amino acid sequence was confirmed by N-terminal amino acid sequencing and amino acid analysis of the overproduced protein. The complete polypeptide chain has 272 amino acid residues and has a calculated Mr of 29,380. E. coli shikimate dehydrogenase is homologous to the shikimate dehydrogenase domain of the fungal arom multifunctional enzymes and to the catabolic quinate dehydrogenase of Neurospora crassa.