The purification of shikimate dehydrogenase from Escherichia coli.
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Chaudhuri S, Coggins JR
The purification of shikimate dehydrogenase from Escherichia coli.
Biochem J. 1985 Feb 15;226(1):217-23.
- PubMed ID
- 3883995 [ View in PubMed]
- Abstract
A procedure was developed for the purification of shikimate dehydrogenase from Escherichia coli. Homogeneous enzyme with specific activity 1100 units/mg of protein was obtained in 21% overall yield. The subunit Mr estimated by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate was 32 000. The native Mr, estimated by gel-permeation chromatography on a TSK G2000SW column, was also 32 000. E. coli shikimate dehydrogenase is therefore a monomeric NADP-linked dehydrogenase.