Site-directed mutants of the cAMP receptor protein--DNA binding of five mutant proteins.
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Gent ME, Gartner S, Gronenborn AM, Sandulache R, Clore GM
Site-directed mutants of the cAMP receptor protein--DNA binding of five mutant proteins.
Protein Eng. 1987 Jun;1(3):201-3.
- PubMed ID
- 3333845 [ View in PubMed]
- Abstract
Oligonucleotide-directed mutagenesis was employed to generate mutants of the cAMP receptor protein (CRP) of Escherichia coli. The mutant proteins were purified to homogeneity and tested for stability and DNA binding. It is shown that mutations at the position of Arg180 abolish specific DNA binding, whereas those at the position Arg185 have very little effect. Both positions have previously been implicated as crucial for the specific interaction between CRP and DNA. The Ser128----Ala mutant shows a slight reduction in DNA binding affinity relative to wild-type. All mutants investigated show similar stability profiles to wild-type CRP with respect to thermolysin proteolysis as a function of temperature.