Neopullulanase 1
Details
- Name
- Neopullulanase 1
- Synonyms
- 3.2.1.135
- Alpha-amylase I
- TVA I
- Gene Name
- tvaI
- Organism
- Thermoactinomyces vulgaris
- Amino acid sequence
>lcl|BSEQ0010976|Neopullulanase 1 MIKLLKPMSLSILLVFILSFSFPFPTAKAAANDNNVEWNGLFHDQGPLFDNAPEPTSTQS VTLKLRTFKGDITSANIKYWDTADNAFHWVPMVWDSNDPTGTFDYWKGTIPASPSIKYYR FQINDGTSTAWYNGNGPSSTEPNADDFYIIPNFKTPDWLKNGVMYQIFPDRFYNGDSSND VQTGSYTYNGTPTEKKAWGSSVYADPGYDNSLVFFGGDLAGIDQKLGYIKKTLGANILYL NPIFKAPTNHKYDTQDYMAVDPAFGDNSTLQTLINDIHSTANGPKGYLILDGVFNHTGDS HPWFDKYNNFSSQGAYESQSSPWYNYYTFYTWPDSYASFLGFNSLPKLNYGNSGSAVRGV IYNNSNSVAKTYLNPPYSVDGWRLDAAQYVDANGNNGSDVTNHQIWSEFRNAVKGVNSNA AIIGEYWGNANPWTAQGNQWDAATNFDGFTQPVSEWITGKDYQNNSASISTTQFDSWLRG TRANYPTNVQQSMMNFLSNHDITRFATRSGGDLWKTYLALIFQMTYVGTPTIYYGDEYGM QGGADPDNRRSFDWSQATPSNSAVALTQKLITIRNQYPALRTGSFMTLITDDTNKIYSYG RFDNVNRIAVVLNNDSVSHTVNVPVWQLSMPNGSTVTDKITGHSYTVQNGMVTVAVDGHY GAVLAQ
- Number of residues
- 666
- Molecular Weight
- 74295.435
- Theoretical pI
- 5.03
- GO Classification
- Functionsmetal ion binding / neopullulanase activityProcessescarbohydrate metabolic processComponentsextracellular region
- General Function
- Neopullulanase activity
- Specific Function
- Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Also hydrolyzes cyclodextrins.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Secreted
- Gene sequence
>lcl|BSEQ0002764|2001 bp ATGATCAAATTATTAAAGCCGATGTCTTTGTCCATCCTCCTTGTCTTCATTCTCTCATTT TCTTTTCCATTTCCCACGGCAAAGGCGGCCGCCAATGACAACAATGTGGAGTGGAATGGA TTGTTTCATGATCAAGGCCCGCTTTTTGACAACGCGCCGGAGCCCACTTCAACCCAGAGT GTCACGCTTAAACTGCGAACCTTCAAAGGAGATATTACCAGCGCCAATATCAAGTATTGG GATACTGCCGACAATGCCTTTCACTGGGTGCCCATGGTTTGGGATTCCAATGACCCGACA GGCACGTTTGATTACTGGAAAGGGACGATCCCGGCAAGCCCATCGATCAAGTATTACCGT TTCCAAATTAACGATGGCACAAGCACGGCCTGGTACAACGGCAATGGCCCTTCTTCCACC GAACCGAATGCCGATGATTTTTATATTATTCCCAATTTCAAAACGCCGGACTGGCTGAAA AACGGCGTAATGTACCAGATCTTTCCGGATCGTTTCTACAATGGCGATTCTTCAAATGAT GTCCAGACAGGCTCCTATACCTATAACGGCACCCCAACGGAGAAAAAAGCATGGGGCAGC AGCGTTTACGCTGATCCGGGGTACGACAATAGTTTGGTCTTTTTTGGCGGAGACCTGGCG GGAATCGATCAAAAACTGGGCTATATCAAAAAGACTCTCGGCGCGAACATCCTCTATCTG AATCCCATCTTCAAGGCGCCAACCAACCACAAATACGACACCCAGGACTACATGGCGGTT GATCCCGCGTTTGGCGACAATTCCACCTTGCAAACGCTGATCAACGACATACACAGCACC GCGAACGGCCCTAAAGGGTACCTTATTTTGGACGGGGTGTTCAACCACACGGGAGACAGC CATCCGTGGTTTGACAAATACAATAATTTTTCGTCTCAGGGAGCATACGAGTCACAATCC AGCCCATGGTACAACTACTATACTTTCTACACATGGCCCGATTCATACGCTTCTTTTCTC GGATTCAACAGTTTGCCCAAACTAAACTACGGCAACAGCGGTTCAGCCGTACGGGGCGTA ATCTACAACAACAGCAATTCGGTGGCTAAAACCTATTTAAACCCGCCTTATTCTGTCGAT GGTTGGCGGCTCGATGCAGCACAATATGTTGACGCAAATGGCAACAATGGAAGTGACGTG ACCAACCACCAGATTTGGAGCGAATTTCGGAATGCCGTCAAGGGAGTGAATTCAAACGCC GCAATCATCGGGGAGTATTGGGGCAACGCCAATCCATGGACGGCCCAGGGAAACCAGTGG GACGCAGCCACCAACTTTGATGGCTTCACCCAGCCTGTTTCTGAATGGATTACCGGCAAA GACTACCAAAACAATTCGGCTTCGATCAGCACCACACAGTTTGACAGTTGGCTTAGGGGG ACACGGGCCAACTACCCTACCAATGTCCAACAGTCCATGATGAACTTCCTTTCCAATCAT GACATCACCCGCTTTGCCACACGTTCCGGCGGAGATCTTTGGAAAACCTACTTGGCCCTG ATCTTTCAGATGACTTATGTGGGCACTCCGACCATTTACTATGGGGACGAATACGGCATG CAGGGAGGCGCGGACCCCGACAACCGCCGGTCCTTTGATTGGTCACAAGCCACTCCCTCC AATAGCGCGGTGGCCCTGACCCAAAAGCTGATCACCATTCGCAACCAATACCCTGCTTTA AGAACCGGTTCCTTTATGACATTAATCACGGACGACACCAACAAAATTTATTCTTATGGG CGTTTTGACAACGTCAACCGAATCGCCGTCGTGCTAAACAATGACTCCGTCAGCCATACG GTCAACGTGCCGGTTTGGCAACTCAGCATGCCAAATGGCAGCACTGTCACGGACAAAATT ACCGGCCATAGTTACACCGTACAAAACGGCATGGTTACCGTTGCCGTTGACGGGCACTAC GGCGCTGTCTTGGCACAATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q60053 UniProtKB Entry Name NEPU1_THEVU GenBank Protein ID 1648826 GenBank Gene ID D13177 - General References
- Tonozuka T, Mogi S, Shimura Y, Ibuka A, Sakai H, Matsuzawa H, Sakano Y, Ohta T: Comparison of primary structures and substrate specificities of two pullulan-hydrolyzing alpha-amylases, TVA I and TVA II, from Thermoactinomyces vulgaris R-47. Biochim Biophys Acta. 1995 Sep 27;1252(1):35-42. [Article]
- Kamitori S, Abe A, Ohtaki A, Kaji A, Tonozuka T, Sakano Y: Crystal structures and structural comparison of Thermoactinomyces vulgaris R-47 alpha-amylase 1 (TVAI) at 1.6 A resolution and alpha-amylase 2 (TVAII) at 2.3 A resolution. J Mol Biol. 2002 Apr 26;318(2):443-53. [Article]