Molecular cloning of human epidermal transglutaminase cDNA from keratinocytes in culture.
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Yamanishi K, Liew FM, Konishi K, Yasuno H, Doi H, Hirano J, Fukushima S
Molecular cloning of human epidermal transglutaminase cDNA from keratinocytes in culture.
Biochem Biophys Res Commun. 1991 Mar 29;175(3):906-13.
- PubMed ID
- 1673840 [ View in PubMed]
- Abstract
We have isolated a cDNA encoding human epidermal transglutaminase, a key enzyme of terminal differentiation of keratinocytes. A cDNA library from cultured human keratinocytes was screened by a PCR-amplified partial cDNA fragment of the enzyme with oligonucleotide primers based on the homology of the transglutaminase family. The cDNA is 2734 bp coding a protein of 817 amino acids. The several regions including the active site cysteine residue are highly conserved among the transglutaminase family. However, the charged N-terminal domain is unique to the epidermal transglutaminse, suggesting that the region is involved in the function of the enzyme in keratinocytes.