[Establishment of transgenic cell line CHL-3A4 and its metabolic activation].
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Chen Q, Wu J, Yu Y
[Establishment of transgenic cell line CHL-3A4 and its metabolic activation].
Zhonghua Yu Fang Yi Xue Za Zhi. 1998 Sep;32(5):281-4.
- PubMed ID
- 10322772 [ View in PubMed]
- Abstract
OBJECTIVE: To establish a model cell line CHL-3A4 to study metabolic pathways for some genotoxic chemicals. METHODS: Complimentary DNA (cDNA) of cloned cytochrome P450-3A4 gene in human liver tissue was transferred to Bluescript M13 vector with reverse transcription polymerase chain reaction (RT-PCR) and DNA recombinant techniques. Restriction endonuclease map analysis and sequencing of partial cloned fragment proved that CYP3A4 cDNA was cloned into Bluescript M13 vector. Then, recombinant expression plasmid pREP9-3A4 was constructed in eukaryotic cell and transfected into Chinese hamster CHL cells. RESULTS: It was proved that a CHL-3A4 transgenic cell line was established, which could lead metabolic activation for aflatoxin B1 (AFB1), sterigmatocystin (STC) and cyclophosphamide (CPA). CONCLUSION: The CHL-3A4 cell line established did express human cytochrome P450 3A4 and could lead metabolic activation for three genotoxic chemicals AFB1, STC and CPA.