Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
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Satomura T, Shimura D, Asai K, Sadaie Y, Hirooka K, Fujita Y
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
J Bacteriol. 2005 Jul;187(14):4813-21.
- PubMed ID
- 15995196 [ View in PubMed]
- Abstract
During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases -13 to -56; +1 is the transcription initiation base determined on primer extension analysis of glsA-glnT) in which a direct repeat, TTTTGTN4TTTTGT, is present. Furthermore, the glsA and glnT genes were biochemically verified to encode glutaminase and glutamine transporter, respectively.