SVH-B interacts directly with p53 and suppresses the transcriptional activity of p53.
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Zhou X, Yang G, Huang R, Chen X, Hu G
SVH-B interacts directly with p53 and suppresses the transcriptional activity of p53.
FEBS Lett. 2007 Oct 16;581(25):4943-8. Epub 2007 Sep 21.
- PubMed ID
- 17904127 [ View in PubMed]
- Abstract
We previously reported that inhibition of SVH-B, a specific splicing variant of SVH, results in apoptotic cell death. In this study, we reveal that this apoptosis may be dependent on the presence of p53. Co-immunoprecipitation and GST pull-down assays have demonstrated that SVH-B directly interacts with p53. In both BEL-7404 cells and p53-null Saos-2 cells transfected with a temperature-sensitive mutant of p53, V143A, ectopically expressed SVH-B suppresses the transcriptional activity of p53, and suppression of SVH by RNA interference increases the transcriptional activity of p53. Our results suggested the function of SVH-B in accelerating growth and inhibition of apoptosis is related to its inhibitory binding to p53.