Structure and cell cycle-regulated transcription of the human cyclin A gene.
Article Details
- CitationCopy to clipboard
Henglein B, Chenivesse X, Wang J, Eick D, Brechot C
Structure and cell cycle-regulated transcription of the human cyclin A gene.
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5490-4.
- PubMed ID
- 8202514 [ View in PubMed]
- Abstract
Cyclin A is a cell cycle regulatory protein that functions in mitotic and S-phase control in mammalian somatic cells. Its deregulated expression may have a role in cellular transformation. We have cloned and sequenced the human cyclin A gene and cDNAs representing its mRNAs and have characterized its promoter. Using synchronized cultures of NIH 3T3 cells stably transfected with cyclin A promoter/luciferase constructs, we show that the promoter is repressed during the G1 phase of the cell cycle and is activated at S-phase entry. Cell cycle regulation of the cyclin A gene promoter is mediated by sequences extending from -79 to +100 relative to the predominant transcription start site. It does not require the presence of a functional retinoblastoma protein.