A common precursor for the two subunits of the penicillin acylase from Escherichia coli ATCC11105.
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Oliver G, Valle F, Rosetti F, Gomez-Pedrozo M, Santamaria P, Gosset G, Bolivar F
A common precursor for the two subunits of the penicillin acylase from Escherichia coli ATCC11105.
Gene. 1985;40(1):9-14.
- PubMed ID
- 3005131 [ View in PubMed]
- Abstract
Penicillin acylase (PA) is an industrial enzyme that is used to convert penicillin G into a precursor for semisynthetic penicillins. We have cloned a segment of DNA that codes for the two subunits required for PA activity. We also report the nucleotide sequence of a DNA fragment that codes for (i) the small subunit, (ii) the N-terminal region of the large subunit and (iii) a putative connecting peptide. These results confirm the existence of a common precursor for both peptides.