Molecular cloning and chromosomal assignment of human calbindin-D9k.
Article Details
- CitationCopy to clipboard
Howard A, Legon S, Spurr NK, Walters JR
Molecular cloning and chromosomal assignment of human calbindin-D9k.
Biochem Biophys Res Commun. 1992 Jun 15;185(2):663-9.
- PubMed ID
- 1610358 [ View in PubMed]
- Abstract
Human calbindin-D9k, the vitamin D-dependent calcium binding protein, has been cloned and sequenced following initial amplification of intestinal cDNA sequences by the polymerase chain reaction using mixed oligonucleotide primers. The derived amino acid sequence of 79 residues has a calculated molecular weight of 9015 and is 89% homologous with the bovine and porcine sequences. Probing DNA from human-rodent somatic cell hybrids mapped human calbindin-D9k to chromosome Xp. A single abundant mRNA transcript was detectable in proximal small intestine but not in kidney, uterus or placenta.